Modifying Enzymes
Worthington Biochemical Corporation ELASTASE 25 MG
Elastase, Suspension, Code: ES, 2X Crystallized, Supplied as an aqueous suspension, Suitable for the Isolation of Type II lung cells, Store at 2 deg. to 8 deg.C, The diluted enzyme should be 0.22u filtered before use, 25mg
Cellscript Inc Cap-Clip™ Acid Pyrophosphatase | 100 units
For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer.
Worthington Biochemical Corporation Trypsin Inhibitor, Soybean, Purified, 100mg
Source: Soybean. Animal Free/AF. Chromatographically purified as described by Frattali, V., and Steiner, R.: Biochem., 7, 521 (1968), modified by Worthington. A dialyzed, lyophilized powder. Purity >90% checked using SDS PAGE. Store at 2-8°C.
Worthington Biochemical Corporation Cellulase CELF, ≥25 units per mg dry weight, 10g
Cellulase CELF, ≥25 units per mg dry weight, 10g, A partially purified, lyophilized powder. Store at 2-8°C.
Apexbio Technology LLC Lambda Exonuclease(Synonyms: Lambda Exo, Lambda exonuclease enzyme, λ exonuclease, λ exonuclease enzyme), 5000U.
Lambda Exonuclease is a DNA-specific exonuclease enzyme that progressively degrades double-stranded DNA in a 5 3 direction preferentially initiating digestion at 5 -phosphorylated termini It displays minor activity toward single-stranded DNA and double-stranded DNA with non-phosphorylated ends but cannot start cleavage at internal nicks or gaps Lambda Exonuclease is commonly employed in molecular biology laboratories for the generation of single-stranded DNA templates DNA single-strand conformation polymorphism (SSCP) analysis selective degradation of linear plasmid forms while preserving supercoiled structures and rolling-circle amplification protocols
Apexbio Technology LLC Phusion high-fidelity DNA polymerase, 100U
Phusion high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, Phusion DNA polymerases produce PCR products with very high speed and accuracy. Also, Phusion DNA polymerases are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. Phusion high-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes. Other sizes are also available. Please inqury us for quote.
Sigma Aldrich Fine Chemicals Biosciences LIPASE FROM CANDIDA RUGOSA 10G
NC3450504 LIPASE FROM CANDIDA RUGOSA 10G
New England Biolabs, Inc. Terminal Transferase – 2500 units
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.
New England Biolabs, Inc. Thermostable Inorganic Pyrophosphatase – 1250 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2.
DESERT BIOLOGICALS RESEARCH Macerozyme R10
Standardized at 3000 maceration units/gram.The activity is based on measuring the volume of single cells released from potato tuber disks. pH: 5.0-6.0
New England Biolabs, Inc. T4 Polynucleotide Kinase (3' phosphatase minus) – 1000 units
T4 Polynucleotide Kinase catalyzes the transfer and exchange of P from the gamma position of ATP to the 5 -hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3'-monophosphates. This modified version exhibits full kinase activity with no 3' phosphatase activity.
Abcam Amsacrine, DNA topoisomerase II inhibitor, 10MG
MW 393.5 Da, Purity >98%. DNA topoisomerase II inhibitor. Antineoplastic agent. Intercalating planar polycyclic aromatic molecule which is active against a number of tumours in mice. Active in vivo..
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Catalase from Aspergillus Niger | 9001-05-2 | MFCD00081483 | 25mg
Catalase from Aspergillus Niger | 9001-05-2 | MFCD00081483 | 25mg
Sigma Aldrich Fine Chemicals Biosciences Cellulase from Trichoderma reesei ATCCÂ 26921 lyophilized powder, >=1 unit/mg solid | 9012-54-8 | MFCD00081510 | 10KU
Cellulase from Trichoderma reesei ATCCÂ 26921 lyophilized powder, >=1 unit/mg solid | 9012-54-8 | MFCD00081510 | 10KU